Why does my DNA or RNA sample float out of the well when loading it onto an agarose gel?
DNA samples purified using the PCR Purification System, Gel DNA Extraction System, etc. or RNA samples purified using the RNA Purification System, DNA free RNA System, etc. may float out of the wells due to carryover of residual ethanol from the final wash step.
Use either of the following steps to remove residual ethanol:
A. Re-purify the sample using a NucleoPur spin column.
B. Dry down the sample in a vacuum centrifuge, and resuspend the pellet in DNA/RNA free water.
Use either of the following steps to remove residual ethanol:
A. Re-purify the sample using a NucleoPur spin column.
B. Dry down the sample in a vacuum centrifuge, and resuspend the pellet in DNA/RNA free water.