What happens if I forget to add the Ethanol to the wash buffer?
First, add the appropriate volume of ethanol to the wash buffer concentrate.
Then simply re-purify the sample using a NucleoPur spin column and appropriate amount of buffer . For example if you are using the PCR Purification System and you mixed 100uls of PCR purification buffer to a 25ul PCR product and washed with 200ul wash buffer concentrate you would have a total volume of 325ul. Simply add the appropriate volume of PCR Purification Buffer (2 volumes in this case = 650ul) and spin through NucleoPur column and proceed with the wash step.
Then simply re-purify the sample using a NucleoPur spin column and appropriate amount of buffer . For example if you are using the PCR Purification System and you mixed 100uls of PCR purification buffer to a 25ul PCR product and washed with 200ul wash buffer concentrate you would have a total volume of 325ul. Simply add the appropriate volume of PCR Purification Buffer (2 volumes in this case = 650ul) and spin through NucleoPur column and proceed with the wash step.